1% hydrogen peroxide increases germination rate and speed

I’ve seen posts here, discussing the use of H2O2 to help seeds germinate. It seems (unless I missed it) that no one’s certain about the best concentration to use. I found a journal article, published in 2021, that answers the question:

Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa

If you prefer a Youtube version:

Improved Cannabis Seed Germination with Hydrogen Peroxide

The purpose of using H2O2 is to sterilize the seeds. Other chemicals can be used but must be washed off, so H2O2 is easier.

The researchers soaked 6-month-old seeds in a dark room overnight at room temperature, using 0%, 1%, 3%, 5%, and 10% solutions. For the next three days, they recorded the number of seeds that had sprouted, poured the liquids out, and replaced them with fresh liquids. Results:

The 1% solution produced the highest germination rate (83%) on day 1. Those 1% seeds also did better than the others when transferred to soil.

The researchers also tested 1% H2O2 vs. sterile water on two strains of 5-year-old seeds:

The benefits of 1% H2O2 for old seeds are dramatic.

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Cool post :metal: thanks for sharing

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They are using straight H2O2?

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Is this straight or diluted with water? I would think it would not make a dif if diluted since you are adding water.

I use the 3% and use a small bowl with RO, maybe a 10:1 water/3% ratio and see tails on day 2

I looked and cannot find a 1% hydrogen peroxide online.

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My thinking as well, 3% splash in a shot glass. I’m close :green_heart::metal:t2:

And what is Finola, Google won’t tell me :person_shrugging:t2:

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95% water 5% peroxide

Has worked for me in the past.

Thinking about going direct in soil from the start, next season.

Did a test this year direct soil and had similar success as starting in water.

Happy growing

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Thanks and same to ya :green_heart::green_heart:
:dash::dash::point_right:t2:

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I’m unsure why the optimum concentration is unclear to some, given my post’s title, text, and figures. :thinking:

The researchers tested several concentrations and the results show that 1% worked best.

It’s true that off-the-shelf H2O2 is usually a 3% solution. Mixing it with water in a 2:1 ratio (say, 2 pints of water plus 1 pint of H2O2) yields a 1% solution.

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So do you think it can be assumed (probably not) that if you water your seed that you planted direct in soil, you would get faster germination if you watered with a 1% hydrogen peroxide, or 5%. Or the soil would just equalize and no difference? Guess I can test.

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Distilled or R/O only when using peroxide. Any solids present will dissipate it immediately. Putting peroxide in soil will do the same.

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I use a basic on tap filter from my tap and get better results with peroxide and would say most tap water should have next to no solids either way.

Also I used to use distilled water, though I found tap water improved germination time.

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@Myfriendis410 and @StevOz have raised good points about the water that should be used. The researchers used “sterile” water, which is the type used in preparing liquids for injection into humans.

@StevOz: Do you de-gas your tap-filtered water?

My house has a water-softener and I expect the dissolved salt and additives aren’t great for plants. I have only a few, so buying distilled water (as @Myfriendis410 suggests) is practical.

Two points from the article that deserve emphasis are:

(1) The seeds sprouted in 1% H2O2 also did better later, when they were transferred to soil

(2) The 10% solution was lethal.

If you’re mixing 3% peroxide with water in that ratio, your concentration is ((0.05 * 0.03) / (1 - 0.05 * 0.03) = ) 0.0015, or 0.15%.

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Never tried H2O2, straight tap water every time :love_you_gesture:

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Correction, i just give it a quick squeeze of the peroxide bottle, theres no exact measurements.

Also I use distilled water.

Happy gardening.

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I got it from one of y’all last year when I started the water soak method. And haven’t looked back :green_heart::metal:t2:

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I think that was me.

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Some of us aint too bright I guess.

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Very well could have been :+1:t2:
In-between bumping the head on the often, the green and the beer i ain’t got a clue :person_shrugging:t2::green_heart::metal:t2:
Just did it with an Auto shortly after the above post :sunglasses:

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I must remind myself that short-term memory can be dicey when dealing with stoners. :face_with_spiral_eyes: :grin:

Bergman, p. 27 says hard water contains beneficial nutrients. That might be why @StevOz gets better germination with tap water than distilled.

I intend to follow the researchers’ procedure, using 1%, from now on. If I get seeds that don’t sprout anyway, I’ll know I gave them the best chance possible, given our current knowledge.

I realized that the water that goes to the hose bibs outside our house doesn’t pass through our water softener. (Duh.) So I’ll use hose-bib water for now.

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The ole lady just got a jug of 12% h2o2 for other reasons.
But reading the back of it says great for seed germanation :+1:t2::green_heart::metal:t2:

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