Your call. If you’re wanting to do a transfer, sooner rather then later. If your wanting to use it to innoculate something, now is as good a time as any.
Fridge will slow growth. Make sure you use gas permiable tape.
Never made an lc that wasnt contaminated. Im bad to ask.
2x a day is ok during colonization of lc as long as you’re not too vigorous with your shakes. Just enough to break up the clumps and incorporate a little O2, which will help with quicker colonizing. If you have a magnetic stirrer, they work great on lc’s. Get the pill spinning just enough to make a vortex and let it buck for a minute or two. Does not hurt to go longer if you have big clumps.
That colored Agar Fs with my head 
For my learning process, why are you stirring your LC before use?
You got some some good rhizo stuff goin on, that’s awesome 
, if ya can try and take a couple transfers and see how they do so you can save the genetics, and you can get those magnetic stirers off Amazon pretty cheap I have one for my rdwc setup, I agree with cory on the advice, LC is something I haven’t tried just yet but will soon. If ya do get good results with transfers you can keep one of them in the fridge for later use 
Ok I took a bunch of transfers again trying to refine things down, took 3 transfers from p3 ( a pin I took from og plate 3) as that looks super strong so I’ll let them grow out a bit about silver dollar size then I’ll be able to tell if there clean or not, one of these will go in the fridge for storage and one to grain to test out the third is just a backup, I also took another transfer from each of the t1’s I still have out , those are also for back up as I have yet to prove out any of these till my jars fully colonize, but are showing progress. now I have several stored in fridge in ziplock sandwich baggies, I guess those are sterile out of the box, makes it easier to pull out and inspect. So now I’m starting a collection 
, not sure how long the plates are good in fridge so don’t know about long term yet. Stay lit y’all 
I assume he’s trying to speed up the colonizing process?
LC blobs grow pretty fast 
You can keep your plates in the fridge for quite a long time. Year plus for sure if they are clean and well sealed 
For long term storage you will want to make up slants.
I have slants that are going on 10 yrs and still clean and viable
I am 
, this sh!ts pretty cool
Cryovials is a tek, looks interesting and simple , distilled water in vial , pc, drop agar chunk in. That’s about it. Can store at room temp as long as there’s not huge temp swings otherwise in fridge. Myc eats up what’s left of agar it’s on then with no food or GE it goes dormant I guess but good for long term storage. Some have revived them recently from 3+ years ago with good success. Just an FYI. Stay lit y’all
So I have some mycelium on some agar cups with a sealed lids in the fridge now. I’d like to do more transfers with those before I just use agar to grain inoculation. I have two lc broth jars. One I cut the inside tissue of a mush and dropped two tiny pieces into the broth. I have the magnet stirring thing and the pills in them. So after I transferred yesterday I stirred. I did another in the evening. Today I’ll do again twice. I want that jar full of clouds so I can withdraw many syringes. Then, if I need to inoculate a lc broth with one to keep the genetics going so I never have to buy more. I’d like to transfer a bunch of the ryno mycelium off that one agar to keep that good strong growth. As far as the agar color it was like 3-4 drops of blue food coloring. Also be able to transfer that to a broth and make lc with it. I duno what hole I’m in but I’m deep in trying to figure out how to never have to purchase another lc syringe. As far as going inoculation of grain to grow I’ll be waiting till fall. But wanted to get a stock pile for when I’m ready. I just finished off a fifth flush off one cake. An still more pins growing with zero signs of contam. Super stoked. I may take another tissue transfer off a mush tonight an transfer to the lc broth and see what happens. Or should I take a chunk of that good ryno mycelium off rhe agar cup and put in the lc broth?
Well, I don’t have much experience with this stuff yet but I would probably take a sample of a mush and do th lc with that if ya like then I would transfer that rhizo to another dish and see if it isolates it more, seems to take with what little I’ve done 1 or 2 transfers at least before it shows more rhizomorphic tendency then do a transfer to broth. But I’m no expert.
I started looking at Li (liquid innoculant) , loos like a very simple process, dh2o, either a media bottle or cryovials . Take you demineralized water and fill container leaving a bit of space at the top, loosely cap and pc at 15 psi for 15 minutes, id need to figure out how long with an insta pot but would guess 30-45 minutes would work fine then in a sab uncap and take your clean plate dice up the myc like you would doing transfers and dump it in, cap tight and that’s it, if doing vials just drop one chunk in instead of the whole plate. Shake or don’t shake doesn’t matter. These can be stored without refrigerating if ya like as long as you don’t have huge temp swings other wise refridgerate. Now you can do say 2 media bottles one as master and the other you can use to noc up grain jars only need to pour 1-10 ml over the grain and that’s it. You do need to give the Li about a week before using. If I get a good candidate I’ll definitely be trying this as it gives you long term storage. Some more fyi , stay lit y’all
Fridges that we store food in are disgusting vectors for contamination. If you are serious about storage, get a mini fridge for your mycological tissue culture preservation.
Will save on space too, but yes I agree with the contamination in a fridge 
Update on new transfers, the 3 transfers from pin 3 plate are just starting to show a little growth and no contamination as far as I can see anyway , so let em grow out a bit more
T2 plates also about the same, these are from my t1 plates that looked promising
These are my backups, looks like a couple may have bacterial spots going on?
Then the second pin , also looks promising so may take a couple transfers from that this weekend
Just a note I am using colored agar it’s not contamination
Yup, did 2 pin plates and have taken 3 transfers from each. One backup, one to run for testing and one to possibly store. That sound about right?
Ok so a quick fyi north spore sells millet but the description doesn’t say whether it’s hulled or unhulled so I emailed them to check and it is unhulled, I assumed it was but now it’s verified
