Nutrient broth:
75ml (1/3 cup) distilled H2O
1 ml Karo
PC 20 min/15 psi
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Sterilized syringe:
Place, in pieces, into a mason jar. Put lid on upside down.
PC 20 min/15 psi
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LC jars (2x 300 ml jars):
Vented - used within a year
Non-vented - master jar
600 ml H2O (distilled)
24 ml Karo (4%)
1.5 g Soy Peptone
PC: 20 min/15 psi
Inoculation: 1-2 ml/300 ml jar
Incubation: 82-84°F for 10-15 days. You should notice growth by then.